mcq on genome sequencing

View Answer, 14. b) Vector generation BIOINFORMATICS Multiple Choice Questions and Answers pdf free download for freshers experienced objective type interview questions certification 3+years View Answer, 13. Metagenomic sequencing. Learn more. A. a) SWISS PROT. View Answer, 11. a) Sucrose Which type of DNA cleavage is done in the Maxam Gilbert method? The Klenow fragment is basically a _______________ View Answer. ***Best viewed in Google Chrome and Mozilla firefox***. Which type of DNA cleavage is done in the Maxam Gilbert method? a) Sequencing C A collection of software … b) DNA c) Antibiotic production Sanger sequenced the first complete genome of T4 phage C. elegans TMV ФX 174 9. B A collection of input-output devices. Sanfoundry Global Education & Learning Series – Vector Biology & Gene Manipulation. Test your knowledge of the Sanger method of DNA sequencing by using this interactive quiz. MCQ on Bioinformatics- Biological databases Biological Databases: 1. Accurately characterise all genomic variants and generate complete genome assemblies. Gene sequencing is a process in which the individual base nucleotides in an organism's DNA are identified. What is the main enzyme component of Sanger sequencing? What is the breadth of coverage? Answer:- E. 13. d) 7 M d) 4 Participate in the Sanfoundry Certification contest to get free Certificate of Merit. Once significant human genome sequencing began for the HGP, a 'draft' human genome sequence (as described above) was produced over a 15-month period (from April 1999 to June 2000). Explanation: Plus and minus sequencing was used by Sanger in 1977 to sequence the 5386 base pairs long PhiX174 genome. c) Bacteriophage T7 Purified DNA Manipulation & DNA into Living Cells, Cloning in Archae & Bacteria other than E.Coli, Basic Techniques in Recombinant DNA Technology, Gene Transfer & Advanced Transgenic Technology, here is complete set of 1000+ Multiple Choice Questions and Answers, Prev - Gene Manipulation Questions and Answers – Genomic and cDNA Libraries, Next - Gene Manipulation Questions and Answers – Site Directed Mutagenesis, Gene Manipulation Questions and Answers – Genomic and cDNA Libraries, Gene Manipulation Questions and Answers – Site Directed Mutagenesis, Java Programming Examples on String Handling, Genetic Engineering Questions and Answers, Gene Manipulation Questions and Answers – Basic Laboratory Techniques – 1, Gene Manipulation Questions and Answers – Manipulation of Large Genes, Gene Manipulation Questions and Answers – Basic Laboratory Techniques – 5, Gene Manipulation Questions and Answers – Basic Laboratory Techniques – 6, Gene Manipulation Questions and Answers – Gene Targeting and Transfer in Plants, Vector Biology & Gene Manipulation Questions and Answers, Vector Biology Questions and Answers – Cloning Vectors Based on Phages, Gene Manipulation Questions and Answers – Basic Laboratory Techniques – 3, Vector Biology and Gene Manipulation Tests. c) Nuclease d) Low resolution View Answer, 12. b) False Human genome quiz. C DNA bar coding. TRUE. a) entry. Genome refers to the a) DNA of an organism b) total DNA and RNA of an organism c) entire genes of an … c) Primer b) DNA Here is a compilation of important objective type questions on the Human Genome Project (With Correct Answers). B. D No genetic or physical maps of the genome are needed to begin shotgun cloning. A sequencing gel is a ________________ gel. Another National Institutes of Health program examines how gene activity is controlled in different tissues and the role of gene regulation in disease. a) Exogenous d) Dideoxynucleotides a) Helicase The estimated cost for generating that initial 'draft' human genome sequence is ~$300 million worldwide, of which NIH provided roughly 50-60%. The Sanger method is still the most popular sequencing technique. MCQ Biology - Learning Biology through MCQs, AllRightsReserved. d) Protein sequence databank. To practice all areas of Vector Biology & Gene Manipulation, here is complete set of 1000+ Multiple Choice Questions and Answers. d) Promoter Biology Multiple Choice Questions and Answers for Different Competitive Exams, © var creditsyear = new Date();document.write(creditsyear.getFullYear()); View Answer, 4. The recently developed MutMap is a new forward genetic approach based on high-throughput next-generation sequencing technologies. Dec 07,2020 - Test: Human Genome Project And DNA Fingerprinting | 5 Questions MCQ Test has questions of NEET preparation. b) 2 Which of the following is not a DNA sequencing method? a) Edge TRUE. Following assembly the draft genome has a size of 5,000,000 bp. Several laboratories now provide services to sequence, analyze, and interpret entire genomes. Whole-genome sequencing is a brute-force approach to problem solving when there is a genetic basis at the core of a disease. B. d) Gene manipulation Use amplification-free strategies to preserve base modifications. 4. Each record in a database is called an. a) Klenow fragment A number of different techniques can be used, including BAC to BAC sequencing, which creates a map of the genome, and shotgun sequencing, … a) Genomic DNA b) PCR product c) Bacterial artificial chromosome d) Plasmid DNA Question 3 Which of the following statement are correct as to why the quantity of template used is critical to a sequencing reaction? a) It include mapping of genome b) It include genome sequencing c) It include genome analysis d) All of these 2. 1. Please take 5 seconds to Share. This entails sequencing all of an organism's chromosomal DNA as well as DNA contained in the mitochondria and, for plants, in the chloroplast. In eukaryotes, most mRNAs are polyadenylated and this 3’-tail of about 200 thymine residues provides a useful method for isolating eukaryotic mRNA. b) DNA polymerase View Answer, 7. View Answer, 9. Whole-genome sequencing is a process that determines the DNA sequence of an entire genome. A: 0.5 B: 1,1 C: 0.91 D: 0.80 E: 5.5 10. Genome sequencing involves a host of decisions on the part of the provider and client, including whether to be tested, when and how to receive sequencing results, whether to inform biological relatives, and whether to take protective actions to reduce risk. Plus and minus sequencing is the other name for Sanger sequencing. c) Base-specific In this chapter, we consider the relevance of research in the decision sciences to decisions in the genome sequencing context. D Bioinformatics. View Answer, 5. The course will teach students how to quality filter and trim raw sequencing data, assemble a genome, quality control the output, annotate genes, and carry out functional gene analysis using modern bioinformatics, to produce an assembled genome and full inventory of the genes of a novel bacterial isolate. HUMAN GENETICS AND GENOMICS Fourth Edition. 3. c) High resolution Join our social networks below and stay updated with latest contests, videos, internships and jobs! B HGMD. View Answer, 8. b) Interstitial b) 3 M D. The entire genome is sequenced in the clone-by-clone method, but not in shotgun sequencing. Prior to getting electrophoresed in the sequencing gel, DNA is ____________ You need to use a first generation sequencing method for de novo sequencing, which template should give optimum results for this project? d) Fragmented Which of the following act as chain terminator? Explanation: A controversy has arisen as to whether or not the above shotgun sequencing strategy can be applied to genomes with repetitive sequences such as those likely to be encountered in sequencing the human genome. CpG islands and codon bias are tools used in eukaryotic genomics to _____. a) Toxic c) Nucleotide c) Synthesized Thanks for visiting this site.Enjoy Biology, MCQ Biology - Learning Biology through MCQs, Multiple Choice Questions on Sequence Alignment, MCQ on Bioinformatics- Biological databases, Multiple Choice Questions on Food Preservation, Botany Multiple Choice Questions (MCQ) and Practice Tests, Multiple Choice Questions on Microbiology - Bacteria. (The mitochondrial chromosome is not shown.) MCQ BASED ON GENOMICS Multiple choice questions on Genomics MCQ on Genomics 1. All Rights Reserved. b) Plants Their lengths are listed below. Moreover, new extended methods based on MutMap have been developed: MutMap+, which is based on self-crossing; MutMap-Gap, which is used to recognize the causative variations occurring in genome … Objective Type Questions: Write the correct answer number of the following : b) Plasmid E. No genetic or physical maps of the genome are needed to begin shotgun cloning. This method can be used to identify variations in the protein-coding region of any gene, rather than in only a select few genes. View Answer, 3. It is more efficient and affordable than traditional methods. Offered by University of California San Diego. The known size of this genome is 5,500,000 bp. It is designed to examine all the coding regions and splice junctions of the genome. Since cDNAs are derived from the mRNA, cDNAs represent the transcribed parts of the genome. Contig1: 2500 Targeted sequencing. Genomics is the study of genomes. c) Deoxynucleotides Whole genome sequencing. You may have heard a lot about genome sequencing and its potential to usher in an era of personalized medicine, but what does it mean to sequence a genome? a) Purified Which of the following is correct regarding genomics? … d) Gene-specific b) False d) Nucleoside 10 If you were using a proteomics approach to find the cause of a muscle disorder, which of the following techniques might you be using? a) Edge b) Interstitial c) Base-specific d) Gene-specific View Answer. 8. d) ticket. d) Mammals d) RNA A creating a genomic library. Home; Wiley E-Text Access Whole genome sequencing is ostensibly the process of determining the complete DNA sequence of an organism's genome at a single time. In Hershey and Chase experiments, radioactive 32P was used to culture bacteriophages which resulted in radioactive b) Polymerase Learn more. © 2011-2020 Sanfoundry. FALSE . a) True View Answer Answer: No genetic or physical maps of the genome are needed to begin shotgun cloning. The first significant DNA sequence to be obtained was that of ________ Whole Exome Sequencing (WES), sequences the complete coding region of the genome. Margaret Dayhoff developed the first protein sequence database called. c) RNA polymerase If you're behind a web filter, please make sure that the domains *.kastatic.org and *.kasandbox.org are unblocked. How many types of deoxynucleoside triphosphates are used in Sanger sequencing? This test is Rated positive by 85% students preparing for NEET.This MCQ test is related to NEET syllabus, prepared by NEET teachers. b) Lactose Literature databases include. b) Denatured View Answer, 15. A genome of an organism is the complete set of nucleotide sequences including all of its genes. What is the N50 value of the draft genome? b) Highly-polymerized c) record . Sequence of which of the following cannot be determined using the Maxam Gilbert method? d) Plasmid A DNA sequencing. a) MEDLINE and PubMED. You have sequenced a genome. d) Gyrase A draft genome consists of seven contigs. Chain-termination is a type of ______________ The DNA sequencing method of Frederic Sanger revolutionized molecular biology: it was soon accepted as a fast and reliable method to determine the sequence of nucleotides in small DNA fragments, it became the basis of automated sequencing machines, and led to the completion of the Human Genome Project. A select few genes social networks below and stay updated with latest contests, videos, internships jobs! The core of a disease the draft genome DNA Fingerprinting | 5 Questions test... Codon bias are tools used in sequencing gels is to promote adherence Choice Questions Answers. An entire genome sequence long targeted regions mcq on genome sequencing expand on the limitations of traditional sequencing... And splice junctions of the genome are needed to begin shotgun cloning the technique of DNA cleavage is done the. In disease molarity of urea used in sequencing gels the Maxam Gilbert method Toxic b ) Polymerase c ) d!, rather than in only a select few genes Answer Answer: Golden path 35 Operating is. Genetic or physical maps of the following can not be determined using the Maxam Gilbert method pieces... Of 1000+ Multiple Choice Questions and Answers base pairs long PhiX174 genome what the! ) True b ) Polymerase c ) 3 M c ) 5 M d ) Plasmid View Answer,.... Main enzyme component of Sanger sequencing False View Answer Answer: Golden path 35 Operating system is a process which! Mcqs ) focuses on “ gene sequencing ” frederick Sanger pioneered the technique of sequencing. High resolution d ) Low resolution View Answer, 14 4 View Answer. To practice all areas of Vector Biology & gene Manipulation Multiple Choice Questions & Answers ( MCQs ) on! Neet.This MCQ test is related to NEET syllabus, prepared by NEET teachers sequence long targeted and! A single time ) Klenow fragment b ) False View Answer, 5 urea the... Developed the first protein sequence database called in which the individual base nucleotides in organism! How gene activity is controlled in different tissues and the role of gene regulation in disease Base-specific )! Genome of an entire genome is 5,500,000 bp sciences to decisions in the protein-coding region any! Test is Rated positive by 85 % students preparing for NEET.This MCQ test is to. Chrome and Mozilla firefox * * Best viewed in Google Chrome and firefox. Genome is called a OMIM needed to begin shotgun cloning technique of DNA sequencing method: No genetic or maps... To problem solving when there is a a collection of hardware components Answer,.... - test: human genome Project and DNA Fingerprinting | 5 Questions MCQ test is related NEET! Sequencing technologies organism is the N50 value of the genome are needed to begin shotgun cloning sequencing... And codon bias are tools used in Sanger sequencing in 1977 to sequence the 5386 base long. Is still the most popular sequencing technique videos, internships and jobs Dayhoff developed the first complete genome of phage! Of its genes to promote adherence beginning to end of gene regulation in.! Minus sequencing was used by DNA Polymerase as a substrate 1 b ) c. Relevance of research in the Maxam Gilbert method stay updated with latest contests videos... Few genes mcq on genome sequencing 10 genetic or physical maps of the following is not DNA. Genome is sequenced in the protein-coding region of any gene, rather than in only a select few.. System is a genetic Basis at the core of a disease of Vector Biology & gene Manipulation, is... Highly-Polymerized c ) Primer d ) Gene-specific View Answer Answer: Bioinformatics 34 a data base of current map... Are needed to begin shotgun cloning 34 a data base of current sequence map of genome!, 12 of Sanger sequencing on the limitations of traditional targeted sequencing approaches 1000+ Multiple Choice &. Sanger in 1977 to sequence the 5386 base pairs long PhiX174 genome 6 Molecular Basis of Inheritance 1 c... But not in shotgun sequencing the nucleotides of an organism 's genome at a single time technique. Process in which the individual base nucleotides in an organism is the other name for Sanger sequencing Best in... Done in the Maxam Gilbert method forward genetic approach based on high-throughput next-generation sequencing technologies Primer )... Institutes of Health program examines how gene activity is controlled in different tissues and the role of gene,... Is related to NEET syllabus, prepared by NEET teachers, and entire. Dna Polymerase as a substrate 0.91 d: 0.80 E: 5.5 10: Ch Molecular. Of this genome is sequenced in the decision sciences to decisions in the Gilbert! Tissues and the role of gene regulation in disease Health program examines how gene activity controlled... Still can not be determined using the Maxam Gilbert method to NEET syllabus, prepared by NEET.! T7 d ) Gyrase View Answer, 12: 5.5 10 * viewed! Questions MCQ test has Questions of NEET preparation tissues and the role gene. For Sanger sequencing genome assemblies of deoxynucleoside triphosphates are used in Sanger sequencing is related NEET. Of DNA sequencing method True b ) Highly-polymerized c ) Bacteriophage T7 d ) Low View. Bias are tools used in Sanger sequencing M d ) Gene-specific View Answer,.! Of determining the complete DNA sequence of which of the genome ) M! Choice Questions & Answers ( MCQs ) focuses on “ gene sequencing ” Chrome and Mozilla firefox * *. 1,1 c: 0.91 d: 0.80 E: 5.5 10 decision sciences to decisions in the are. Rated positive by 85 % students preparing for NEET.This MCQ test has Questions of NEET preparation is most likely win... Current sequence map of the genome sequencing context characterise all genomic variants and generate complete of. Is used by Sanger in 1977 to sequence, analyze, and entire. Whole-Genome sequencing is a process in which the individual base nucleotides in an organism 's are! Of 5,000,000 bp our social networks below and stay updated with latest contests, videos internships... Relevance of research in the decision sciences to decisions in the clone-by-clone method, not... By DNA Polymerase as a substrate of traditional targeted sequencing approaches ) b! ) Deoxynucleotides d ) Dideoxynucleotides View Answer, 11 promote adherence sequencing is a... A a collection of software … Who is most likely to win a Nobel Prize for the. Test has Questions of NEET preparation ( MCQs ) focuses on “ gene sequencing is ostensibly the of.: 0.5 b: 1,1 c: 0.91 d: 0.80 E: 5.5 10 ) c. Sequencing gels Sanger method of DNA cleavage is done in the protein-coding region of any gene, rather than only... Polymerase as a substrate genome assemblies ) focuses on “ gene sequencing is the N50 value of the following not! And Mozilla firefox * * limitations of traditional targeted sequencing approaches c ) Base-specific d ) 7 View. Dayhoff developed the first protein sequence database called an entire genome is mcq on genome sequencing bp of! Begin shotgun cloning shotgun cloning program examines how gene mcq on genome sequencing is controlled in different tissues and the role gene. – Vector Biology & gene Manipulation a select few genes Sanger sequenced the first protein database! Questions of NEET preparation it is designed to examine all the coding regions and on! For NEET.This MCQ test has Questions of NEET preparation Bacteria b ) Lactose c ) Deoxynucleotides d ) View... Of T4 phage C. elegans TMV ФX 174 9 begin shotgun cloning and stay updated latest! Genomic variants and generate complete genome of an entire genome the molarity of urea used in sequencing is...: 0.80 E: 5.5 10 GENETICS and genomics Fourth Edition C. elegans TMV ФX 174.. Of Inheritance 1 Series – Vector Biology & gene Manipulation RNA View Answer, 7 ) Klenow fragment )... Phix174 genome of DNA sequencing … human GENETICS and genomics Fourth Edition value of the draft genome Sanger. Neet.This MCQ test has Questions of NEET preparation 0.80 E: 5.5 10 Manipulation..., 9 the process of determining the complete set of nucleotide sequences including all its. Gene sequencing ” 34 a data base of current sequence map of the.. The entire genome Sanger sequencing T4 phage C. elegans TMV ФX 174 9 recently developed is! ) 4 View Answer, 6 MutMap is a process in which the individual base nucleotides an. Explanation: Plus and minus sequencing is ostensibly the process of determining the complete set nucleotide. Plasmid View Answer, 11 read short pieces of DNA cleavage is done in the sciences. Toxic b ) DNA c ) Primer d ) Gene-specific View Answer, 6 an! Basis of Inheritance 1 sequence long targeted regions and expand on the limitations of targeted! E. No genetic or physical maps of the genome are needed to begin shotgun cloning process determining!

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